h漫无码动漫av动漫在线播放,偷偷藏不住电视剧免费播放完整版,亚洲日本va中文字幕久久,亚洲精品成人无码中文毛片

銷售熱線

19126518388
  • 技術(shù)文章ARTICLE

    您當(dāng)前的位置:首頁 > 技術(shù)文章 > 成功轉(zhuǎn)染脂肪間充質(zhì)干細(xì)胞(ADMSCs)(IF6.684)文章分享

    成功轉(zhuǎn)染脂肪間充質(zhì)干細(xì)胞(ADMSCs)(IF6.684)文章分享

    發(fā)布時間: 2023-02-24  點擊次數(shù): 1049次

    zeta life  Advanced DNA RNA 轉(zhuǎn)染試劑轉(zhuǎn)染脂肪間充質(zhì)干細(xì)胞(ADMSCs)(IF6.684)文章分享圖片8
    zeta life轉(zhuǎn)染脂肪間充質(zhì)干細(xì)胞(ADMSCs)(IF6.684)文章分享編輯

    一、轉(zhuǎn)染犬/狗脂肪間充質(zhì)干細(xì)胞(ADMSCs)siRNA基因沉默 簡述

     熒光素標(biāo)記的siRNA(小干擾 RNA)每個基因設(shè)計有三個siRNA,使用美國Zeta Life公司Advanced DNA RNA轉(zhuǎn)染試劑(Zeta Life,Menlo Park,CA,United States)用siRNA轉(zhuǎn)染犬/狗脂肪間充質(zhì)干細(xì)胞(ADMSCs),RT-qPCR用于檢測siRNA 的沉默效率,并選擇沉默效率高的一種,在整個轉(zhuǎn)染過程中,該基因每7天轉(zhuǎn)染一次以保持沉默。

    二、轉(zhuǎn)染犬/狗脂肪間充質(zhì)干細(xì)胞(ADMSCs)基因過表達(dá) 簡述

    使用美國Zeta Life公司Advanced DNA RNA轉(zhuǎn)染試劑(Zeta Life,Menlo Park,CA,USA)轉(zhuǎn)染AAV-293細(xì)胞收集重組腺病毒顆粒并感染犬/狗脂肪間充質(zhì)干細(xì)胞(ADMSCs),用 RT-qPCR和蛋白質(zhì)印跡檢測目的基因的表達(dá)。

    三、培養(yǎng)條件 簡述

     脂肪間充質(zhì)干細(xì)胞(ADMSCs),使用美國Zeta Life公司z7010FBS-500胎牛血清培養(yǎng)。

    四、Adenovirus-Mediated Gene Overexpression原文分享

    圖片10 

    zeta life轉(zhuǎn)染脂肪間充質(zhì)干細(xì)胞(ADMSCs)(IF6.684)文章分享編輯

    The cDNA sequences for genes were synthesized by Wuhan Gene Create Biological Engineering Co., Ltd. (Wuhan, China). The cDNA for each gene was PCR-amplifified and inserted into pAdTrack-CMV previously digested with BglII/HindIII using the In-Fusion HD cloning kit (Takara, Japan) (Sleight et al., 2010). The resulting plasmid was linearized with PmeI, and then homologous recombination was performed with pAdeasy-1 in E. coli BJ5183. The recombinant plasmid with PacI linearization was used to transfect AAV-293 cells to

    produce recombinant adenovirus particles using the Advanced DNA RNA transfection reagent (Zeta Life, Menlo Park, CA, United States). RT-qPCR (Nolan et al., 2006) and Western-blot (following the Elabscience Western blot detection kit operation manual) were used to detect the expression of target gene. The canine ADMSCs were transfected with the recombinant adenovirus particles.

     

    五、siRNA-Mediated Gene Silencing原文分享

    圖片11 

    zeta life轉(zhuǎn)染脂肪間充質(zhì)干細(xì)胞(ADMSCs)(IF6.684)文章分享編輯

     

    Fluorescein-labeled siRNA . Each gene was designed with three siRNAs. In the process of transdifffferentiation, the canine ADMSCs were transfected with siRNAs using the Advanced DNA RNA transfection reagent (Zeta Life, Menlo Park, CA, United States). RT-qPCR (Nolan et al., 2006) was used to detect the silencing effiffifficiency of siRNAs and the one with the highest silencing effiffifficiency was selected. The gene was silenced every 7 days throughout the transdifffferentiation to keep it silent.

    六、美國Zeta Life 公司與美國加利福尼亞大學(xué)舊金山校區(qū)聯(lián)合開發(fā)用于哺乳動物細(xì)胞、活體動物轉(zhuǎn)染的 Advanced DNA RNA 第三代多肽小分子轉(zhuǎn)染試劑,此技術(shù)成為新的全球蛋白功能、免疫細(xì)胞及干細(xì)胞治療、研發(fā)及生產(chǎn)的主要關(guān)鍵技術(shù)之一。

     

    七、文獻(xiàn)中所用轉(zhuǎn)染試劑產(chǎn)品信息

    產(chǎn)品名稱:Advanced DNA RNA 轉(zhuǎn)染試劑

    貨號:AD600025、AD600050、AD600075、AD600150

    規(guī)格:0.25ML、0.5ML、0.75ML、1.5ML

     

     

     

產(chǎn)品中心 Products
性高湖久久久久久久久aaaaa| 97无人区夜夜夜爽涩日本无码 | 国产97色在线 | 日韩| 色狠狠色噜噜AV天堂一区| 精品国产不卡一区二区三区| 亚洲AV无码专区亚洲AV伊甸园| 成人性生交大片免费看| 三a级做爰片免费观看| 国产国产国产国产系列| 欧美乱强伦XXXXX高潮| 《色诱女教师》在线观看| 他揉捏她两乳不停呻吟人妻| 欧美大成色WWW永久网站婷| 欧美大荫蒂毛茸茸视频| 日韩人妻无码一区二区三区| 香港三级精品三级在线专区| gogogo日本免费观看视频| 一本到高清视频免费观看| 欧美孕妇变态重口另类 | 国产日产亚洲系列| 精品久久久久中文字幕| 亚洲中文字幕无码中文字在线 | 性xxxx视频播放免费| 国产伦孑沙发午休精品 | 国产免费av片在线无码免费看| 久久久久久久久久久国产| 日韩蜜桃一区二区三区| 国内精品久久人妻无码hd| 永久免费av无码网站性色av| 强伦姧人妻波多野洁衣| 97免费人妻在线视频| 国产产无码乱码精品久久鸭 | 粗大猛烈进出高潮视频免费看| 最美情侣在线播放观看视频免费| 日本最大但人文艺术第一组| 熟妇人妻无乱码中文字幕| 国产成人精品日本亚洲专区61| 国产欧美精品一区二区三区| 人妻AV无码一区二区三区| 国产美女被遭强高潮免费网站| 亚洲AV成人无码天堂|